Table 1 Antibodies and immunohistochemical staining conditions
From: Female breast cancer classification using immunohistochemistry biomarkers staining in Botswana
Antibody | Clone | Control | Retrieval Buffer | Dilution | Incubation time at RT |
|---|---|---|---|---|---|
Oestrogen Receptor | NCL-L-ER6 F11 | Breast cancer | EDTA pH 8 | 1:75 | 60 min |
Progesterone Receptor | NCL-L-PGR-312 | Breast cancer | EDTA pH 8 | 1:50 | 60 min |
c-erbB-2 | Polyclonal | Confirmed breast cancer | EDTA pH 8 | 1:200 | 60 min |
Ki67 | MIB-1 | tonsil | EDTA pH 8 | 1:50 | 60 min |
CK 5/6 | D5/16 B4 | tonsil | Tris–EDTA pH 9 | 1:50 | 60 min |
EGFR | NCL-L-EGFR-384 | placenta | Tris–EDTA pH 9 | 1:50 | 60 min |