Table 1 Primer sequences designed for qPCR
Genes | Sequence | Tm | CG% |
|---|---|---|---|
MMP2 | F-AGTGGATGATGCCTTTGCTC | 60 °C | 50.0% |
R- GAGTCCGTCCTTACCGTCAA | 55.0% | ||
CDH-1 | F-CAGCACGTACACAGCCCTAA | 59.9 °C | 55.0% |
R- ACCTGAGGCTTTGGATTCCT | 60.1 °C | ||
Bax | F- GACGGCCTCCTCTCCTACTT | 59.8 °C | 60.0% |
R- CTCAGCCCATCTTCTTCCAG | 59.9 °C | 55.0% | |
Bcl-2 | F-GGATTGTGGCCTTCTTTGAG | 59.7 °C | 50.0% |
R- GCCGGTTCAGGTACTCAGTC | 60.0% | ||
GAPDH | F-CGAGATCCCTCCAAAATCAA | 60.0 °C | 45.0% |
R- TTCACACCCATGACGAACAT | 59.8 °C | 45.0% |