Fig. 11

Induction of apoptosis in ATC cells by Gyp LI. (A-C) Flow cytometry analysis showing the distribution of viable, early, and late apoptotic cells in the lower left, lower right and upper right quadrants, respectively. The apoptosis rate was calculated based on the percentage of apoptotic cells. Treatment with 40 and 60 µM Gyp LI significantly enhanced apoptosis in ATC cells. (D-G) Western blot analysis of ATC cell lysates post-treatment with Gyp LI. The expression levels of HSP90AA1, CASP3, cleaved CASP3, CASP9, and cleaved CASP9 were assessed, with GAPDH used as a loading control