Fig. 4

RNF157 overexpression promotes CC cell proliferation. A Western blotting and detection of RNF157 expression in Huh7 cells after transfection of the overexpression sequence. B OE-control protein sequencing KEGG functional enrichment. C CCK-8 assay was used to assess the proliferation level of Huh7 cells. D A plate colony formation assay was performed to detect the proliferation level of Huh7 cells. E Images of tumours removed from nude mice four weeks after the subcutaneous injection of Huh-7 cells (n = 5). The tumour weights of the two groups of nude mice were measured. Tumour diameter was used to plot tumour volume curves. The images are from one representative experiment. The data are expressed as the means ± SDs of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001). Ordinary one-way ANOVA with multiple comparisons testing was used to examine the statistical significance of differences among the three independent groups