Fig. 2

PRDX1 knockdown facilitates erastin-induced ferroptosis in DLBCL cells. (A) Western blot analysis was performed to assess PRDX1 protein expression in OCI-Ly3 and DB cells treated with or without erastin. (B) Cell viability was measured by CCK-8 assay in OCI-Ly3 and DB cells transfected with si-NC (negative control siRNA) or si-PRDX1 and treated with or without erastin. (C) Lipid peroxidation and ferroptosis-related markers, including MDA levels, intracellular iron levels, and GSH content, were measured in OCI-Ly3 and DB cells transfected with si-NC or si-PRDX1, with or without erastin treatment. (D) Protein levels of ferroptosis-related markers, including COX2 and GPX4 were quantified by western blot in OCI-Ly3 and DB cells transfected with si-NC or si-PRDX1 and treated with or without erastin. ^*P < 0.05, ^**P < 0.01 vs. DMSO group or si-NC (erastin) group