Fig. 1

Knockdown of PRDX1 inhibits DLBCL cell proliferation, migration, and invasion, while enhancing apoptosis. A. PRDX1 expression was analyzed in patients with DLBCL in the GEPIA2 database. The red box represents tumor samples, and the blue box represents normal samples. B. Using the GSE95290 dataset from the Oncomine database, PRDX1 expression was detected in DLBCL tissues. The scatter plot shows PRDX1 expression in each sample, with red dots representing cancer samples and blue dots representing normal samples. C. PRDX1 expression in DLBCL cell lines (OCI-Ly3 and DB) was quantified by RT-qPCR. ^*P < 0.05, ^**P < 0.01 vs. normal or GM12878 group. D-E. Transfection efficiency was confirmed by RT-qPCR and western blot following siRNA transfection. F. Using the CCK-8 assay, growth curves were generated based on OD450 after PRDX1 knockdown. G. Flow cytometry analysis with Annexin V-FITC and PI staining was used to determine the cell apoptosis level after PRDX1 knockdown. H-I. The evaluation of cell migration and invasion following PRDX1 knockdown was conducted via the Transwell assays. ^**P < 0.01 vs. si-NC group