Fig. 4

hLRH1pS510 is critical for cell viability in LUSC cells. (A) The construct of hLRH1 expression vector. EF-1α, elongation factor-1α; Fl, Flag; IRES, internal ribosome entry site. (B) Western blot analysis for the indicated proteins in the revealed LK-2 and BERF cells. (C) Representative phase contrast and GFP images in the indicated cell lines. Scale bar, 100 μm. (D) The relative levels of cell viability in the indicated cells are shown in the histograms (mean ± SD, n = 8)