Fig. 8

MP-HJ-1b Reduces SLC31A1 Expression and Suppresses Proliferation, Invasion, and Migration Abilities of LN229 Cells. (A) Significant reduction in SLC31A1 protein expression in LN229 cells after treatment with MP-HJ-1b. (B) Immunofluorescence Validation of the effect of MP-HJ-1b on SLC31A1 Protein Expression. The results demonstrate that both LN229 cell group and over-expression cell group exhibit decreased expression of SLC31A1 protein after treatment with MP-HJ-1b. Each bar represents the mean ± SEM of three independent experiments. Compared to over-expression, #### p < 0.0001; compared to Con, *p < 0.05. (C) Transwell assay demonstrates the inhibitory effect of MP-HJ-1b on the invasion ability of LN229 cells. (D) Scratch assay indicates a notable decrease in the migration ability of cells treated with MP-HJ-1b. (E) Colony formation assay shows that MP-HJ-1b significantly inhibits the colony formation ability of LN229 cells. Each bar represents the mean ± SEM of three independent experiments. Compared to the over-expression, the over-expression-MP-HJ-1b group exhibits significant statistical differences, ##p < 0.01, ### p < 0.001, #### p < 0.0001; compared to the LN229 group, the LN229-MP-HJ-1b group show significant statistical differences, *p < 0.05, **p < 0.01