Fig. 7

Enhanced Expression of SLC31A1 Gene Promotes Proliferation, Metastasis, and Invasion in GBM. (A) RT-PCR validation of differential SLC31A1 mRNA expression between shRNA-SLC31A1 cells and over-expression cells. (B) Western blot validation of varying SLC31A1 protein expression levels among distinct stable transfection cell lines. (C) Immunofluorescence validation of the Impact of high and low SLC31A1 expression levels on protein expression. The results demonstrate that the fluorescence intensity of individual cells in the SLC31A1 interference group is lower than that of the control group, whereas the fluorescence intensity of individual cells in the SLC31A1 overexpression group is higher than that of the control group. Each bar represents the mean ± SEM of three independent experiments. Compared to the vector group, #### p < 0.0001; compared to shRNA-Con, ****p < 0.0001. (D) CCK-8 assay assessing the influence of SLC31A1 expression levels on cell proliferation in LN229 cells. Reduced SLC31A1 expression leads to a significant decrease in cell proliferation, whereas elevated SLC31A1 expression enhances proliferation. (E) Transwell assay illustrating that SLC31A1 overexpression notably enhances the invasion capability of cells LN229. (F) Scratch assay evaluating the impact of SLC31A1 expression levels on the migration ability of LN229 cells. LN229 cells with heightened SLC31A1 expression exhibit significantly improved migration ability. (G) Colony formation assay confirming the disparity in colony formation ability in LN229 cells due to varying SLC31A1 expression levels. Cells with low SLC31A1 expression display diminished colony formation ability, whereas high SLC31A1 expression fosters colony formation. Each bar represents the mean ± SEM of three independent experiments. Compared to Vector, ##p < 0.01, ### p < 0.001, #### p < 0.0001; compared to shRNA-Con, **p < 0.01, ***p < 0.001, ****p < 0.0001