Fig. 6
a Study design for contrast ultrasound imaging of a mouse. b Study design for ultrasound-induced microbubble destruction and replenishment. c B-mode images from tumor-bearing mice and non-tumor-bearing mice; USMI images obtained with all MBsNRP2 and freely circulated MBsNRP2 before and post the destructive pulse and in tumor-bearing mice and non-tumor-bearing mice. The image panel presents ultrasound B-mode images in grey (upper row) and the respective contrast-enhanced ultrasound image in brown (lower row) including the color-coded USMI dTE signal distribution ((pre-burst)—(post-burst)) in the region of interest (green contour), a local area of the tumor(blue contour), and reference region for USMI(yellow contour). d Study design for attached MBsNRP2. e Quantification of attached MBsNRP2. f CLSM images and colocalization for immunofluorescence localization staining of CD31 and NRP2 in tumor-bearing mice and non-tumor-bearing mice. g Fluorescence intensity quantification of NRP2 in tumor-bearing mice and non-tumor-bearing mice. h Fluorescence intensity quantification of NRP2 expression in tumor-bearing mice and non-tumor-bearing mice. i IHC percentage quantification of NRP2-positive cells in tumor-bearing mice and non-tumor-bearing mice. n = 6 samples per group; *, p < 0.05; **, p < 0.01; ***, p < 0.005; error bars present SD