Fig. 4

a CLSM images and colocalization for immunofluorescence localization staining of CD31 and NRP2. b Schematic diagram of the transwell co—culture. c Fluorescence intensity quantification of NRP2. d NRP2 expression was depleted in HMEC- 1 cells using different prostate cancer cell lines as stimuli. β-actin was used as a loading control. e Flow cytometric analyses of HMEC- 1 cells exposed to different prostate cancer cell lines. f Prostate cancer cells exhibited the ability to promote tube formation in endothelial cells. g Quantifi1cation of tube formation exposed to different prostate cancer cells. n = 5 samples per group; *, p < 0.05; **, p < 0.01; ***, p < 0.005; error bars present SD