Table 1 Primer sequences and reaction conditions for genotyping two Tag SNPs in CDK8
SNPs | Primer sequence (5’→3’) | Wild/Mutated allele | Annealing temperature (°C) | Restriction enzyme | Digest condition | Product size (bp) |
|---|---|---|---|---|---|---|
rs17083838 | F: TCTGTTGCACTTGCCATATCA | G/A | 58 | Msp I | 37 °C | A: 194 |
R: TCCAAAGACTTTCAAAGACACTCA | 45 min | G: 126 + 68 | ||||
rs7992670 | F: TTTCATGCAGATACCACGTCA | G/A | 58 | Mbo II | 37 °C | A: 151 |
R: TGGATTCACGGGTTCGTTAT | 30 min | G: 113 + 38 |