Fig. 4
From: FOXO1 mediates miR-99a-5p/E2F7 to restrain breast cancer cell proliferation and induce apoptosis
miR-99a-5p targeted inhibition of E2F7 expression in breast cancer cells. mRNAs that were upregulated in breast cancer were identified using the GEO database and visualized via volcano plots (A). The target mRNAs of miR-99a-5p that were upregulated in breast cancer and negatively correlated with FOXO1 expression were identified using Venn analysis (B). Dual luciferase reporter assays were used to confirm the binding of miR-99a-5p to E2F7 mRNA (C). The effects of transfecting miR-99a-5p mimics or inhibitors on E2F7 expression in breast cancer cells were measured by RT-qPCR (D) and western blot assays (E). (All n = 3) *p < 0.05 vs. inhibitor NC group; #p < 0.05 vs. mimics NC group