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Fig. 2 | BMC Cancer

Fig. 2

From: FOXO1 mediates miR-99a-5p/E2F7 to restrain breast cancer cell proliferation and induce apoptosis

Fig. 2

FOXO1 facilitates miR-99a-5p transcription. MiRNAs that are downregulated in breast cancer were identified using the GEO database and visualize via volcano plots (A). miRNAs targeted by FOXO1 were screened using the TransmiR online tool, and those downregulated in breast cancer were further with Venn analysis (B). Correlation between the expression of hsa-miR-99a and the overall survival of breast cancer patients was investigated using Kaplan-Meier Plotter (C). ChIP experiment was conducted to confirm the binding of FOXO1 to miR-99a promoter (D) and dual luciferase reporter assays were performed (E). Expression of miR-99a-5p in MCF7 and MDA-MB-231 cells after knockdown or overexpression of FOXO1 was measured by RT-qPCR (F). Correlation between FOXO1 with hsa-miR-99a-5p and hsa-miR-99a-3p was analyzed using the TCGA database (G). (All n = 3) *p < 0.05 vs. sh-NC group, IgG group or Vector group; #p < 0.05 vs. ov-NC group

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BMC Cancer

ISSN: 1471-2407

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