Fig. 11

Knockdown of ADORA2B inhibits the proliferation and migration of HNSC cells. QRT-PCR analysis confirming the knockdown efficiency of ADORA2B in SAS and SCC-9 cells transfected with three independent siRNAs (si-ADORA2B-1, si-ADORA2B-2, si-ADORA2B-3) compared to the negative control (si-NC) (a). CCK-8 assays showing reduced proliferation of SAS (b) and SCC-9 (c) cells following ADORA2B knockdown. Colony formation assays demonstrating a significant decrease in colony-forming ability in SAS (d) and SCC-9 (e) cells upon ADORA2B knockdown. Wound healing assays showing impaired migration rates in SAS (f) and SCC-9 (g) cells after ADORA2B knockdown at 0 h, 6 h, and 9 h. Abbreviations: HNSC, head and neck squamous cell carcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant