Fig. 2

PD-L1-AAV particles reduced PD-L1 expression. PD-L1 expression at the cellular level was determined by western blot analysis. GAPDH was used as a loading control (A). Western blot results were analyzed using the relative quantification (RQ) value, and the knockdown efficiency was calculated as (1‑RQ) ×100 (%). All values are presented as the mean ± SE of three independent experiments. Statistical significance was determined using an unpaired Student’s t-test. ** p < 0.01 (B)