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Table 3 Standard conditions for the PCR amplification reactions

From: A model of basement membrane-related regulators for prediction of prognoses in esophageal cancer and verification in vitro

Step

Temperature

Time

Cycle

Initial denaturation

95 ℃

10 min

1

Denaturation

95 ℃

10 s

40

Primer annealing and extension

60 ℃

30 s

Melting stage

95 ℃

15 s

1

60 ℃

60 s

95 ℃

15 s