Fig. 2

Identification of 12 candidate TAAbs based on multi-omics approach. a UMAP plot of CRC epithelial cells, color-coded by subcelltype subtype. b Heatmap showing the tissue preference of each cell subset as indicated by the odds ratios. c Volcano plot showing significantly upregulated or downregulated genes in each cluster, with the top five markers for each cluster highlighted. d UMAP plot of CRC epithelial cells, color-coded by CMS subtypes. e A four-set venn diagram showing the intersection of four CMS subtypes. f Volcano plot of the differentially expressed proteins between CRC and NC groups. g Upset plot for signature genes from different studies. h GO pathway enrichment analysis of 12 candidate TAAs