Fig. 8

SIM2 knockdown represses cell cycle progression of EC cells in vitro and metastasis of EC cells in vivo. A The effect of SIM2 expression on EC cell cycle progression was detected by flow cytometry. B The expression levels of cleaved caspase 3, Cyclin D1 and CDK4 in HEC-1-A and KLE cell lines with SIM2 knockdown or overexpression, were detected by western blot. C and D HEC-1-A cells were injected into nude mice via tail vein, and H&E staining was used to examine lung (C) and liver (D) metastases in mice, and the severity of metastasis in two groups was compared. For the in vitro assays, Student’s t test was used to compare the data between two groups. For the in vivo assays, Wilcoxon test was used to compare the data between two groups. *P < 0.05, **P < 0.01 and ***P < 0.001