Fig. 7

SIM2 promotes the viability and represses the apoptosis of EC cells. A Western blot was used to detect SIM2 expression levels in different cell lines including hEM15A, HEC-1-A, AN3CA, KLE and Ishikawa. B qRT-PCR was used to detect SIM2 expression levels in HEC-1-A and KLE cell lines with SIM2 knockdown or overexpression. C Western blot was used to detect SIM2 expression levels in HEC-1-A and KLE cell lines with SIM2 knockdown or overexpression. D The effect of SIM2 expression on EC cell viability was detected by CCK-8 assay. E The effect of SIM2 expression on EC cell apoptosis was detected by flow cytometry. Student’s t test was used to compare the data between two groups. *P < 0.05, **P < 0.01 and ***P < 0.001