Fig. 1

Flow cytometry gating strategy. (A) Selection of CD45+ lymphocytes, (B) gating of single cells, (C) excluding lineage positive cells, (D) differentiation of NK cells and ILCs, (E) differentiation of NK cells (CD94+, CD56+) and their subpopulations CD56brightNK cells (CD94+, CD56++) and CD56dimNK cells (CD94+, CD56+), (F) CD16 positive CD56dimNK cells. (G) differentiation of ILCs (CD94−, CD127+) and their subpopulations ILC1-like cells (CD117−, CRTH2−), ILC2 (CD117+/−, CRTH2+) and ILC3 neutral (CD117+, CRTH2−), (H) subdivision of ILC3neutral into ILCPs (NKp44−) and NKp44+ ILC3