Fig. 6

Modulation of cell cycle progression by the members of miR- 23a/24–2/27a cluster. Cell cycle distribution of MCF-7 cells transfected with miR- 23a, miR- 24–2, and miR- 27a (A) After PI staining, flow cytometry of MCF- 7 cells was performed to assess cell cycle progression 24 h post transfection. (B) Cyclin D1 (C) c-Myc expressions were quantified for cell proliferation using western blot. GAPDH was used as a loading control. Data is representative of the mean ± SEM. p-value < 0.05 was considered to indicate significance