Fig. 5

Regulation of Invasion and EMT pathway by miR- 23a, miR- 24–2, and miR- 27a in breast cancer cells. Cell invasion assay was used to assess the invasion capacity in (A) MCF-7, and (B) MDA-MB-231 breast cancer cells after overexpression or downregulation of individual components of miR- 23a/27a/24–2 cluster. The images were captured at 10 × magnification. (C) E- Cadherin, (D) Claudin 1, (E) Keratin 8/18, and (F) Fibronectin expressions were quantified for epithelial-mesenchymal transition using western blot. GAPDH was used as a loading control. Data is representative of the mean ± SEM for three independent experiments. p-value < 0.05 was considered to indicate significance