Fig. 3

Members of miR- 23a/27a/24–2 cluster targets GSK3β. Estimation of miR- 23a, miR- 24–2 and miR- 27a levels upon miR and anti-miR treatments in (A) MCF- 7 and (B) MDA-MB- 231 breast cancer cells using qRT-PCR. (C) Predicted binding sites of miR- 23a/miR- 24–2/miR- 27a in the 3’UTR of GSK3β (D) Dual-luciferase assay to determine the direct binding of miR- 23a/miR- 24–2/miR- 27a in 3’ UTR sequence of GSK3β. Translational quantification of GSK3β (E) in MCF-7, and (F) MDA-MB- 231 cells and (G) β-catenin in MCF-7 cells by western blot analysis 24 h post transfection. GAPDH was used as a loading control. Data is representative of the mean ± SEM of 3 independent experiments. p-value < 0.05 was considered to indicate significance