Table 1 Primer information
Gene | SNP ID | 1st-PCR | 2nd-PCR | UEP |
|---|---|---|---|---|
CHRNA5 | rs16969968 | ACGTTGGATGCACGGACATCATTTTCCTTC | ACGTTGGATGGGTAGTGGACCAAAATCTTC | TTGGAAGCTGCGCTC |
CHRNA5 | rs17486278 | ACGTTGGATGTGAGGAACTGGGAGAATGAC | ACGTTGGATGAAGGAGCAAGGTTACATTTC | GGAGAAAGGTTACATTTCTTTGAAAAT |
CHRNA5 | rs555018 | ACGTTGGATGTTGTTCACTGCTGTTTCCCC | ACGTTGGATGGAGGGTAGATGGAGTTCTTG | CAGATAGTATTTTTGGAGTGCCTATT |
CHRNA5 | rs692780 | ACGTTGGATGATGAAGGGTGAGGGCTCTG | ACGTTGGATGGGGAGCTTAGCGATAAAAGG | ACCTCTCAGACACAATTCAGTGAATTT |
CHRNA3 | rs1051730 | ACGTTGGATGCAGCAGTTGTACTTGATGTC | ACGTTGGATGCTCAAGGACTATTGGGAGAG | CCCCCCCATCATCAAAGCCCCAGGCTA |
CHRNA3 | rs8042374 | ACGTTGGATGGGGACAGGTATAGAGATTTT | ACGTTGGATGCAGGTGCCTGAATTCAAAAT | TTTGTTCCTCACTGTCTT |
CHRNA3 | rs3743078 | ACGTTGGATGAAGGCACTGGAAGATGAGAG | ACGTTGGATGGCCTCAGTATCCCTGTTGGT | GTATCCCTGTTGGTAAATGG |
CHRNA3 | rs12910984 | ACGTTGGATGACAGGTGTAAGCCACTGTGC | ACGTTGGATGGTGACTCATCAGAAAATGGC | GGGGTTGCTTTAAAGACTTTAGGAG |
CHRNA3 | rs1317286 | ACGTTGGATGTCCTCTGGTTGGCTAGTTTG | ACGTTGGATGGCATGGTGGGAGTGAAATTG | GGGAGGTATAAACTCAGCAGGGCACCA |
CHRNA3 | rs938682 | ACGTTGGATGTGCCACTGCCTTTTGTTGTC | ACGTTGGATGAGTGACGGTCACAGCTATTC | CGGCCCAGCTATTCATCTCTGCCC |
CHRNB4 | rs7178270 | ACGTTGGATGAGGACGTGCCAGTGATTTAG | ACGTTGGATGTCAAGTAAACCACCTGCACC | AAACAAGTCCCCATCTTGG |