Fig. 4
From: Radiotherapy is enhanced by CPH:SA IL-1α microparticles in a murine HNSCC tumor model
Radiotherapy depletes the immune cell population in the tumor draining lymph nodes. Inguinal lymph nodes near the irradiated tumors were harvested from a subset of mEERL tumor‐bearing C57Bl/6 mice (n = 4/treatment group) on Treatment Day 3 (1day after the first dose of IL-1α MPs) and Treatment Day 11 (1day after the second dose of IL-1α MPs). Lymph node homogenates were analyzed by flow cytometry for select immune cell subsets i.e. CD45 + cells (A), CD3 + T cells (B), CD4 + T cells (C), CD8 + T cells (D), HPV + CD8 + T cells (E), NK cells (F), and dendritic cells (DCs) (G). Both irradiated and non-irradiated tumors were harvested from a subset of mice from each treatment group. Tumors were homogenized and single cell suspensions were analyzed by flow cytometry for macrophages (H) and DCs (I). Error bars represent standard error from the mean. *p < .05