Fig. 5

Viability of leukemic cells and feeders under individual cultivation and co-cultivation conditions. Analysis of cell number (A, D), metabolic activity (B, E) and 2-DG uptake (C, F) in leukemic cells (SD-1, UPF26K) (A, B, C) and feeders (NHDF, hMSCs) (D, E, F) after 48 h of co-cultivation under NX and HX conditions. Data are shown as box and whisker plots: boxes represent the IQR, the median (horizontal line), minimum and maximum values (whiskers) and individual data points (dots) are plotted. The numbers above the boxplots indicate the mean for each group. The control condition (NX individual cultivation in MIX = N × SD-1 or NX UPF26K or NX NHDF or NX hMSC) set as 100% represents the individual cultivation of particular cell type cultivated in a MIX medium under NX. Statistical analyses were performed using a nonparametric, two-tailed Mann–Whitney U test for two-sample comparisons. Statistical significance (p ≤ 0.05) is indicated in the graphs using symbols that denote significant differences between the following comparisons: * = all co-cultivation samples and HX individual samples vs. NX individual cultivation control, # = HX co-cultivation samples vs. HX individual cultivation control, + = different co-cultivation counterparts within the same oxygen level (e.g. SD-1 from NX co-cultivation with NHDF vs. SD-1 from NX co-cultivation with hMSCs), significance brackets = differences for the same sample (i.e., the same cell type and co-cultivation counterparts) grown under NX vs. HX