Fig. 3

Proliferation of NHDF and hMSCs cells under different cultivation conditions in the individual cultivation. Doubling time (A) of NHDF and hMSCs after 48 h of cultivation. Colony formation assay (CFU-F) (B) assesses colony formation efficiency and proliferative potential after 14 days under different cultivation conditions. Bar graphs show individual data points (dots) and SD. The numbers above the bars indicate the mean for each group. Light microscopy images (C) showing size and morphology of NHDF and hMSCs´ colonies (the scale bar in the images represents 200 μm). In (A) and (B), statistical analyses were performed using a nonparametric, two-tailed Mann–Whitney U test for two-sample comparisons. Statistical significance (p ≤ 0.05) is indicated in the graphs using symbols that denote significant differences between the following comparisons: * = all individual cultivation samples vs. NX αMEM individual control medium (for each cell type separately), # = HX individual cultivation samples vs. HX αMEM individual control medium, significance brackets = differences for cells cultivated in the same cultivation medium grown under NX vs. HX