Fig. 1

Proliferation of SD-1 and UPF26K cells under different cultivation conditions in the individual cultivation. (A, B) Proliferation (cell number over time) of SD-1 (A) and UPF26K (B) cells grown in “IMDM” (standard control medium) and a “mix” media (mixture of αMEM and IMDM) under normoxia (NX – 20% O₂) and hypoxia (HX – 1% O₂). Data are shown as box and whisker plots: boxes represent the interquartile range (IQR), the median (horizontal line), minimum and maximum values (whiskers) and individual data points (dots) are plotted. (C) Doubling time of SD-1 and UPF26K cells after 2 days of cultivation (darker bars of appropriate cell type) and after 7 days of preincubation under HX (lighter bars – “HX 7d preincubation”). Bar graphs show individual data points (dots) and standard deviation (SD). The numbers above the boxplots and bars indicate the mean for each group. In (A) and (B), the control condition (“% of ctrl-NX IMDM 24 h”) set as 100%, represents the individual cultivation of particular cell type cultivated in a IMDM medium after 24 h under NX. Statistical analyses were performed using a nonparametric, two-tailed Mann–Whitney U test for two-sample comparisons. Statistical significance (p ≤ 0.05) is indicated in the graphs using symbols that denote significant differences between the following comparisons: * = all individual cultivation samples vs. NX IMDM individual control medium at 24 h, significance brackets = differences for the same sample (i.e., the same cell type and cultivation medium) grown under NX vs. HX