Fig. 9

Functional analysis and validation of ARNTL2. A The correlation of ARNTL2 with functional state in LUAD based on the CancerSEA database is illustrated through an interactive bubble chart. B, C Detailed functional correlations are presented for the LUAD chip (B) and a patient-derived xenograft model (C). D Enrichment analysis was conducted using Gene Set Enrichment Analysis (GSEA) to compare high and low expression levels of ARNTL2 across the TCGA, GSE68465, and GSE31210 databases. E Western blot analysis was performed to assess ARNTL2 protein expression levels in normal lung tissues and the indicated cell lines. F, G A Transwell assay was utilized to evaluate the invasive ability of A549 and H1299 cells following ARNTL2 silencing, with a scale bar of 100 μm. H-J The impact of ARNTL2 silencing on invasion and metastasis in A549 cells was analyzed using a lung metastasis model. The metastatic tumor lesions in each mouse lung were assessed through Hematoxylin and eosin (H&E) staining (H). Representative images of H&E-stained lung sections from mice injected intravenously with the indicated cells are shown (H). The number of lung metastatic nodules (I) and the lung weight of mice (J) in the indicated groups were measured and analyzed. K Western blot analysis was conducted to evaluate the expression levels of the indicated proteins. The target bands are derived from the corresponding region of the original blot images. **p < 0.01, ***p < 0.001