Fig. 5
TRM cells marked by PTGER2 worsen prognosis in H. pylori negative gastric cancer. (A) Venn diagram of shared and differentially expressed genes between T cells of CD8-C4-AREG cluster from patients with different H. pylori infection status. (B) GSEA showing enriched pathways in H. pylori + derived T cells. NES denotes normalized enrichment score. (C) Violin plots and corresponding dots showing that the expression levels of AREG and PTGER2 were upregulated in T cells of CD8-C4-AREG cluster with H. pylori-. (D) Real-time PCR quantification of AREG, PTGER2, and GZMB expression in CD8+T cells under control or following stimulation with H. pylori. The expression levels of each gene were normalized to GAPDH and then adjusted relative to the control group (served as 1). Data shown as mean ± SD. P value was calculated with student’s t test. (E) The multicolor immunofluorescence staining of marker genes with PTGER2, CD8 and AREG in stroma and tumor tissues between different H. pylori infection status. (F) Overall survival for GC patients further stratified according to PTGER2 expression within CD8 expression strata from the TCGA database. P-value was calculated by the log-rank test