Fig. 2

Dissection and identifying infiltrated cell types in gastric cancer tissues. (A) Workflow showing the process of sample collection, single-cell dissociation, sorting, scRNA-seq, bioinformatic analysis and validation. (B) Re-clustering of CD4⁺T cell and CD8⁺T cells. (C) Average expression of selected T cell function-associated genes across different clusters. The box is proportional to the relative expression level of each gene. (D) The developmental trajectory of CD8⁺T cells inferred by Monocle2. Each dot corresponds to one single cell, colored according to its cluster label. (E) Monocle components were correlated with functional features of CD8⁺T cells, including scores of cytotoxicity and exhaustion calculated by the mean expression of gene sets related to T cell status (see Methods). (F) Significantly decreased or increased score of cytotoxicity in the differentiation process colored by cell clusters. The solid lines represent the relationship between the score with Monocle components