Fig. 6

Effect of combination therapy with simvastatin and olaparib on γΗ2ΑΧ expression in androgen-independent or taxane-resistant prostate cancer cells. Cells were incubated in a medium. After 48 h, cells were cultured in a medium with or without simvastatin (PC-3; 2 µM, other cells; 5 µM) and olaparib (10 µM). Proteins were collected after 48 h for PC-3 and LNCaP-LA cells and 96 h for 22Rv1 and 22Rv1-CR cells. Protein expression of γΗ2ΑΧ was evaluated using western blotting. A representative experiment is shown, which was repeated three times with similar results