A genetically encoded fluorescent sensor enables sensitive and specific detection of IDH mutant associated oncometabolite D-2-hydroxyglutarate

Table 1 Summary of D-2-HG or L-2-HG concentrations in biological matrices of glioma patients as presented in current literature

Enantiomer	Tissue Type	IDH Mutant (µM)	IDH Wildtype (µM)	Reference
D	Blood	10.9 2.94	0.8 (0.7–0.9) 1.61	[11, 18]
D	CSF	0.15 (0.05–11.4) 7.4 (0.3–109.0) 3.3 ± 0.5	0.08 (0.05–0.5) 0.4 (0.1–2.6) 2.4 ± 0.3	[9, 10, 11]
D	Tissue	1964.8 (median)	14.0 (median)	[19]
L	Blood	0.41 ± 0.26 1.24	- 1.32	[11, 18]
L	CSF	0.35 (0.01–0.5) 0.9 (0.4–4.1) 2.5 ± 0.3	0.35 (0.1–0.88) 1.0 (0.1–6.1) 2.6 ± 0.23	[9, 10, 11]
Total 2HG	Blood	0.66 ± 0.41 0.89 (0.22–1.91) 0.79 ± 0.36	0.65 ± 0.30 0.85 (0.24–1.87) -	[12, 20, 21]
Total 2HG	CSF	5.9 ± 0.6	5.0 ± 0.5	[10]
Total 2HG	Urine	20.0 (7.0-136.9)	11.6 (5.6–37.5)	[20]
Total 2HG	Tissue	1971.5 (median)	27.0 (median)	[19]

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