Fig. 7

Silencing USP18 reduced tumor growth in vivo by mediating POU4F1/PRKAA2 axis. (A) Establishment of xenograft model. (B-C) Tumor volume and weight of sh-NC, sh-USP18, sh-USP18 + POU4F1, sh-POU4F1, or sh-POU4F1 + PRKAA2 group. (D) Western blot was used to measure protein expression of PRKAA2 in tumor tissues. (E) PRKAA2 protein detection was carried out using IHC in tumor tissues. According to the positive cells (brown), IHC quantification analysis was completed by normalization of sh-NC group to 1. nP < 0.01, mP < 0.001