Fig. 3
From: Establishment of a novel mouse model of colorectal cancer by orthotopic transplantation
Development of various types of small tumors by transplanted Caco-2-mScarlet cells. A Gross view of the cecum one month after the transplantation of Caco-2-mScarlet cells. Small tumor foci expressing mScarlet were visible from the outside of the cecum. B Caco-2-mScarlet cell tumors observed from the luminal side after dissection. C H&E staining of the cecum one month after transplantation of Caco-2-mScarlet cells. Low- (left) and high-magnification (right) images are shown. The dotted line encircles the xenografted region. D Distribution of Caco-2-mScarlet (red) cells in different types of tumors. (left) In the flat monolayer tumor foci, Caco-2 cells (T) were present directly adjacent to the normal epithelial cells (N) that were negative for mScarlet. Caco-2 cells also formed ACF-like foci, which were exclusively composed of mScarlet-positive tumor cells (center), and protruded, pedunculated tumors supported internally by host stromal cells (right). These tumors (T) were also found in proximity to the normal epithelium (N)