Fig. 2
From: Establishment of a novel mouse model of colorectal cancer by orthotopic transplantation
Transplantation of Caco-2 colorectal cancer cells into the cecal epithelium. A Caco-2 cells expressing the fluorescent marker, mScarlet, (Caco-2-mScarlet) were seeded onto the epithelium-removed surface of the cecum. B A magnified view of the cecal surface upon the seeding of Caco-2-mScarlet cells. C, D Immunofluorescence analysis of the cecum one week after the transplantation of Caco-2-mScarlet cells. C mScarlet-positive Caco-2 cells formed many tumor foci within the cecum, which were surrounded by the dense collagen layer. D Proliferating cells were stained by EdU labeling. As it has been demonstrated that CD133 is expressed at the detectable levels in most Caco-2 cells [28, 29], an antibody specific for human CD133 was used to label Caco-2 cells. Many transplanted Caco-2 cells exhibited positivity for EdU