Table 1 Primers sequences and amplicon size of PCR program for MSI detection
Marker | Primer sequences(5’ to3’) | Amplicon size (bp) | Gene |
|---|---|---|---|
BAT-25 | F: TACCAGGTGGCAAAGGGCA R: TCTGCATTTTAACTATGGCTC | 153 | c-Kit |
BAT-26 | F: CTGCGGTAATCAAGTTTTTTAG R: AACCATTCAACATTTTTAACCC | 183 | HMSH2 |
NR-27 | F: AACCATGCTTGCAAACCACT R: CGATAATACTAGCAATGACC | 87 | Inhibitor of apoptosis protein-1 |
NR-21 | F: GAGTCGCTGGCACAGTTCTA R: CTGGTCACTCGCGTTTACAA | 109 | SLC7A8 |
NR-24 | F: GCTGAATTTTACCTCCTGAC R: ATTGTGCCATTGCATTCCAA | 131 | Zinc finger 2 |