Table 1 Primers used for RT-qPCR analysis
From: Prion protein regulates invasiveness in glioblastoma stem cells
Gene | Forward Primer sequence (5’-3’) | Reverse Primer sequence (5’-3’) |
|---|---|---|
ABCB5 | ATTGGAGTGGTTAGTCAAGAGCC | AGTCACATCATCTCGTCCATACT |
CD24 | CTCCTACCCACGCAGATTTATTC | AGAGTGAGACCACGAAGAGAC |
CD44 | ACAGTACAGCCATTTATGCAAGG | TTCCCCACTTCTCAAAACATTCT |
DOT1L | CTGCCGGTCTACGATAAACATC | AGCTTGAGATCCGGGATTTCT |
EGF | TGTCCACGCAATGTGTCTGAA | CATTATCGGGTGAGGAACAACC |
EGFR | CAGACCGGACGACAGGC | ATACTGGACGGAGTCAGGGG |
EPHA4 | ACTTGGAAGGCGTGGTCACT | CCCAGACCCAATGCCACGAA |
EPHB4 | CTCCTTCCTGCGGCTAAACG | GGACGTAGCTCATCTCGGCA |
LAMA5 | GGCTTTCCCCGAGCTGTACT | AGGGTCCCACCGTAGGATGA |
NCAM1 | CAGCCAGTCCAAGGGGAACC | ACGGGAGCCTGATCTCTGGT |
CD133 | ACCAGGTAAGAACCCGGATCAA | CAAGAATTCCGCCTCCTAGCACT |
PRNP after deletion | ATGAGCCGTTGCTAATGCCA | GCCAGAGGTATCCAGGCAAA |
PRNP before deletion | GGGACCCCAGTGAGGAGG | TGCTCTGAAAAGCGAAGCCA |
PRNP in deletion | CCTGGAGGCAACCGCTAC | TCGGCTTGTTCCACTGACTG |
SOCS3 | GCCACCTACTGAACCCTCCT | ACGGTCTTCCGACAGAGATG |
SOX2 | ATGCACCGCTACGACG | CTTTTGCACCCCTCCCATTT |
SOX9 | AGGTGCTCAAAGGCTACGACT | AGATGTGCGTCTGCTCCGTG |
TBP | AGGATAAGAGAGCCACGAACCA | CTTGCTGCCAGTCTGGACTGT |
THBS2 | AGCTCCTCTTCAATCCCCGC | AGGCGTCACCCTCTCCATTG |
TIMP3 | CTTCGGCACGCTGGTCTACA | GCCATCATAGACGCGACCTGT |
WNT10B | GTGAGCGAGACCCCACTATG | CACTCTGTAACCTTGCACTCATC |