Fig. 4

PRNP expression in patient tumor samples predicts lower survival and may be associated with migration and invasion pathways in glioblastoma stem cells. (a) Correlation between PRNP expression and GCS’s regulators obtained from the TCGA database. (b) Curves showing survival probabilities in patients with varying PRNP (right) or CD44 expression (left). Survival and expression data were obtained from Gliovis, using the TCGA GBM dataset. (c-e) correlation analysis between CD44 and PRNP expression in GBM subtype samples. Scatter plot illustrating the relationship between CD44 and PRNP. The line represents the best-fit linear regression, with the Pearson correlation coefficient (r) and significance level (p-value) indicated in the top left corner. (f) t-distributed stochastic neighbor embedding (UMAP) graphic showing GSCs cultivated as spheres (n = 10,536) distributed according to transcription patterns. Different colors represent each. (g) Violin plot shows PRNP expression in each cluster. Central dots represent the means. (h) Gene Set Enrichment Analysis (GSEA) of cluster 10 showing main upregulated pathways. (i-j) Enrichment score of cell migration-related pathways (i) and cell growth-related pathways (j)