Fig. 4

Colon Adenocarcinomas Demonstrated Heterogenous Innate Immune Activation Associated with Tumor Immunogenicity and Immune Cell Exhaustion. A Clustered heatmap of z-scores of the ssGSEA scores of all 5 innate immune pathways in colon adenocarcinomas. B TIMER results of the quantification of immune cell populations within tumors in each of the 3 groups of innate immune activation. C Absolute count and class of each mutation event for the three innate group identified. D Mutations within the protein-coding region of P53, KRAS, APC, and MUC16 were quantified for each innate group and then translated into a ratio of mutated versus non-mutated tumors. Chi-Square test p-values shown above. E RSEM normalized expression values of immune cell exhaustion markers PCDC1, CTLA4, HAVCR2, and LAG3 in each of the three innate immune groups. Symbols: * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001 from a Mann–Whitney U test. Abbreviations: TLR (Toll Like Receptor), CLR (C-type Lectin Receptor), RIG-I (Retinoic acid Inducible Gene I), NOD (Nucleotide Binding Oligomerization Domain), cGAS (Cyclic GMP-AMP Synthase), HR (Hazard Ratio), FDR (False Discovery Rate), RSEM (RNA-Seq by Expectation–Maximization)