Fig. 3



Enhanced stem cell-like features in HCT116 cell strains: Radar Charts showing the overlap of (A) stem cell types (B) Stemness Signatures (C) Transcription Factors between the list of genes of transcriptome data and Stemchecker database. The overlap significance is plotted as (-log10 p-value) represented by squares on the inner side. Outer squares represent the fractional overlap. The statistical details shown in respective lower panel. The significance (p-value) was calculated by the hypergeometric test and the adjusted p-value is calculated by Bonferroni correction. (D) Venn diagram showing the overlap between Transcriptome Stem cell (SC) genes and GSE 44,076 obtained from NCBI-GEO. (E) DIC microscopy images of colonosphere of HCT116 strains (magnification 20X) showing progressive increase in size. Colonosphere diameter was measured using NIS-element-AR software was used to calculate the volume of colonospheres (Upper right panel). (F) qPCR analysis of EMT associated genes. (G) CSC frequency at different time period within the colonosphere of HCT116 strains using flow cytometry. (H) DIC images of colonosphere of HCT116 strains (magnification 20X) in float and (I) after transferring into tissue culture surface 48 h post attachment