Fig. 6

Germ cells show impaired capability of forming follicles in the presence of etoposide. A: Photomicrographs of haemotoxylin and eosin stained E13.5 CD1 ovaries cultured for 6 days either in control medium (Ai) or in medium supplemented with the highest dose of etoposide (150 ng ml⁻¹; Aii). By day 6 of culture, there were significantly fewer germ cells remaining in etoposide-treated ovaries than in controls (b). Arrows denote germ cells within the ovary. Scale bars: both 50 μm. Bars denote mean ± SEM, n = 5 for controls, n = 6 for treatment group. Stars denote significant differences relative to control (***p < 0.0001)