Table 1 List of primers for qRT-PCR. Sequence of primers (forward and reverse) used for qRT-PCR experiments. GenBank accession numbers for each gene are indicated in brackets. The primers were designed using an old version of the software Primer 3.0, available at the following website: http://www-genome.wi.mit.edu/cgi-bin/primer/primer3.cgi/
Name | Sequence 5'-3' (forward and reverse primer) | Product length | Primer nM | Mg2+ mM | Reference |
|---|---|---|---|---|---|
GAPDH* (NM_002046) | GAA GGT GAA GGT CGG AGT C | 226 | 200 | 4 | - |
GAA GAT GGT GAT GGG ATT TC | 200 | ||||
HPRT1* (NM_000194) | TCA GGC AGT ATA ATC CAA AGA TGG T | 84 | 400 | 4 | [38] |
AGT CTG GCT TAT ATC CAA CAC TTC G | 400 | ||||
PBGD* (NM_000190) | CTG CAC GAT CCC GAG ACT CT | 98 | 400 | 4 | [39] |
GCT GTA TGC ACG GCT ACT GG | 400 | ||||
SDHA* (NM_004168) | TGG GAA CAA GAG GGC ATC TG | 400 | 4 | [12] | |
CCA CCA CTG CAT CAA ATT CAT G | 400 | ||||
TBP* (X54993) | CAC GAA CCA CGG CAC TGA TT | 89 | 400 | 4 | [40] |
TTT TCT TGC TGC CAG TCT GGA C | 400 | ||||
BCRP (AF098951) | CAC AAC CAT TGC ATC TTG GC | 74 | 400 | 4 | [41] |
GCT GCA AAG CCG TAA ATC CA | 400 | ||||
DPD (NM_000110) | CCA AAG GCA GTA AAG CAG GAA | 66 | 400 | 4 | [42] |
TCA CGA CTC CCC GTA TCG A | 400 | ||||
EGFR (NM_005228) | TGG TCA AGT GCT GGA TGA TAG A | 156 | 400 | 4 | - |
GGT AGA AGT TGG AGT CTG TAG GA | 400 | ||||
ERCC-1 (NM_001983) | GGG AAT TTG GCG ACG TAA TTC | 71 | 400 | 3 | [43] |
GCG GAG GCT GAG GAA CAG | 400 | ||||
GST-π (NM_000852) | CGG AGA CCT CAC CCT GTA | 169 | 400 | 5 | - |
CGC CTC ATA GTT GGT GTA GA | 400 | ||||
MDR1 (AF016535) | TGG TTC AGG TGG CTC TGG AT | 72 | 300 | 4 | [44] |
CTG TAG ACA AAC GAT GAG CTA TCA CA | 300 | ||||
MLH-1 (NM_000249) | GGC ACA GCA TCA AAC CAA GT | 147 | 400 | 4 | - |
GCA AGC ATG GCA AGG TCA A | 400 | ||||
MRP1 (L05628) | CAA TGC TGT GAT GGC GAT G | 70 | 400 | 4 | [39] |
GAT CCG ATT GTC TTT GCT CTT CA | 400 | ||||
MRP2 (NM_000392) | TGC AGC CTC CAT AAC CAT GAG | 80 | 400 | 3 | [45] |
GAT GCC TGC CAT TGG ACC TA | 400 | ||||
MT-II (NM_005953) | GAT CCC AAC TGC TCC TGC | 127 | 400 | 4 | - |
ACT TGG CAC AGC CCA CAG | 400 | ||||
MVP (NM_017458) | CAG CTG GCC ATC GAG ATC A | 68 | 400 | 4 | [39] |
TCC AGT CTC TGA GCC TCA TGC | 400 | ||||
TP (NM_001953) | CCT TGG ATA AGC TGG AGT CT | 107 | 400 | 4 | - |
CCT ACT CTG ACC CAC GAT AC | 400 | ||||
TS (NM_001071) | CCA GAG ATC GGG AGA CAT GG | 66 | 400 | 4 | - |
TAC GTG AGC AGG GCG TAG CT | 400 | ||||
TOPO I (J03250) | CTC CAC AAC GAT TCC CAG AT | 149 | 400 | 3 | - |
TTA TGT TCA CTG TTG CTA TGC TT | 400 | ||||
TOPO IIα (NM_001067) | GTA ATT TTG ATG TCC CTC CAC GA | 223 | 400 | 3 | - |
TCA AGG TCT GAC ACG ACA CTT | 400 | ||||
TOPO IIβ (NM_001068) | GCA GCC GAA AGA CCT AAA TA | 85 | 400 | 3 | - |
AAT CAT TAT TGT CAT CAT CAT CAT C | 400 |