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Table 2 PCR conditions for sequencing analysis of the C22orf2 gene

From: Is the gene encoding Chibby implicated as a tumour suppressor in colorectal cancer ?

Exon

Primers (5'-3')

Annealing temperature (°C)

PCR product (bp)

2

ex2F: GGCATAAGGTCAGTGATCCAG

58

236

 

ex2R: TTCAGAGACACGCCTCAGCAC

  

3–4

ex3F: GGTCATATTGTTGGCGGAAG

58

490

 

ex4R: CCAAACTGGCATTGAGGAGA

  

5

ex5F: CCAGCAGCATCAGAGAAGTG

56

245

 

ex5R: AAAAGCACTCAGCCACATCC