Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: IL-6 signaling by STAT3 participates in the change from hyperplasia to neoplasia in NRP-152 and NRP-154 rat prostatic epithelial cells

Figure 1

NRP-154 cells, but not NRP-152 cells, have constitutively-activated STAT3. NRP-152 and NRP-154 cells were grown in their respective complete media. For IC flow cytometry, confluent cells were harvested, fixed, permeabilized, and stained for P-STAT3, as described in Materials & Methods. Cells were analyzed on a Becton-Dickinson FACScan for fluorescence in FL2. The thin purple line on the histogram shows the results for NRP-152 cells + testosterone; the thick blue line shows the results for NRP-154 cells minus testosterone. The fluorescent staining of NRP-152 cells plus testosterone was the same as for the fluorochrome control on both cell lines, and the same for NRP-152 cells not treated with testosterone (data not shown). The NRP-154 cells were approximately 10 times more fluorescent than the NRP-152 cells (p < 0.001 by Kolmogorov-Smirnov {KS} statistical analysis). A representative histogram from 4 independent experiments is shown here.

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us